Publication

Free Radical-Initiated Peptide Sequencing Mass Spectrometry for Phosphopeptide Post-translational Modification Analysis

조회수 : 138 등록일 : 2019.04.15 00:00

저자 : Jang, I (Jang, Inae); Moon, B (Moon, Bongjin); Oh, HB (Oh, Han Bin)
출처 : JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
출판일 : 2019.03.15


Free Radical-Initiated Peptide Sequencing Mass Spectrometry for Phosphopeptide Post-translational Modification Analysis


Jang, I (Jang, Inae)1 ] ; Jeon, A (Jeon, Aeran)1 ] Lim, SG (Lim, Suk Gyu)2,3 ] Hong, DK (Hong, Duk Ki)2,3 ] Kim, MS (Kim, Min Soo)2,4 ] Jo, JH (Jo, Jae Hyeong)2,5 ] Lee, ST (Lee, Sang Tak)1 ] Moon, B (Moon, Bongjin)1 ] Oh, HB (Oh, Han Bin)1 ]


[ 1 ] Sogang Univ, Dept Chem, Seoul 04107, South Korea
[ 2 ] Seoul Sci High Sch, Seoul 03066, South Korea
[ 3 ] Seoul Natl Univ, Seoul 08826, South Korea
[ 4 ] Korea Univ, Seoul 02841, South Korea
[ 5 ] Korea Adv Inst Sci & Technol, Daejeon 34141, South Korea



Free radical-initiated peptide sequencing mass spectrometry (FRIPS MS) was employed to analyze a number of representative singly or doubly protonated phosphopeptides (phosphoserine and phosphotyrosine peptides) in positive ion mode. In contrast to collision-activated dissociation (CAD) results, a loss of a phosphate group occurred to a limited degree for both phosphoserine and phosphotyrosine peptides, and thus, localization of a phosphorylated site was readily achieved. Considering that FRIPS MS supplies a substantial amount of collisional energy to peptides, this result was quite unexpected because a labile phosphate group was conserved. Analysis of the resulting peptide fragments revealed the extensive production of a-, c-, x-, and z-type fragments (with some minor b- and y-type fragments), suggesting that radical-driven peptide fragmentation was the primary mechanism involved in the FRIPS MS of phosphopeptides. Results of this study clearly indicate that FRIPS MS is a promising tool for the characterization of post-translational modifications such as phosphorylation.






  • 목록보기
  • 이전글보기
  • 다음글보기