Publication

Nanochannel-Confined TAMRA-Polypyrrole Stained DNA Stretching by Varying the Ionic Strength from Micromolar to Millimolar Concentrations

조회수 : 104 등록일 : 2019.04.15 00:00

저자 : Lee, S (Lee, Seonghyun); Jo, K (Jo, Kyubong)
출처 : POLYMERS
출판일 : 2019.01.15


Nanochannel-Confined TAMRA-Polypyrrole Stained DNA Stretching by Varying the Ionic Strength from Micromolar to Millimolar Concentrations


Lee, S (Lee, Seonghyun)1 ] Lee, Y (Lee, Yelin)1 ] Kim, Y (Kim, Yongkyun)1 ] Wang, C (Wang, Cong)2 ] Park, J (Park, Jungyul)2 ] Jung, GY (Jung, Gun Young)3 ] Chen, YL (Chen, Yenglong)4,5,6 ] Chang, R (Chang, Rakwoo)7 ] Ikeda, S (Ikeda, Shuji)8 ] Sugiyama, H (Sugiyama, Hiroshi)8 ] Jo, K (Jo, Kyubong)1 ]


[ 1 ] Sogang Univ, Dept Chem & Integrated Biotechnol, Seoul 04107, South Korea
[ 2 ] Sogang Univ, Dept Mech Engn, Seoul 04107, South Korea
[ 3 ] GIST, Sch Mat Sci & Engn, Gwangju 61005, South Korea
[ 4 ] Natl Tsing Hua Univ, Acad Sinica, Inst Phys, Taipei 10617, Taiwan
[ 5 ] Natl Tsing Hua Univ, Dept Chem Engn, Taipei 10617, Taiwan
[ 6 ] Natl Taiwan Univ, Dept Phys, Taipei 10617, Taiwan
[ 7 ] Kwangwoon Univ, Dept Chem, Seoul 01897, South Korea
[ 8 ] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068501, Japan



Large DNA molecules have been utilized as a model system to investigate polymer physics. However, DNA visualization via intercalating dyes has generated equivocal results due to dye-induced structural deformation, particularly unwanted unwinding of the double helix. Thus, the contour length increases and the persistence length changes so unpredictably that there has been a controversy. In this paper, we used TAMRA-polypyrrole to stain single DNA molecules. Since this staining did not change the contour length of B-form DNA, we utilized TAMRA-polypyrrole stained DNA as a tool to measure the persistence length by changing the ionic strength. Then, we investigated DNA stretching in nanochannels by varying the ionic strength from 0.06 mM to 47 mM to evaluate several polymer physics theories proposed by Odijk, de Gennes and recent papers to deal with these regimes.






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