Publication

Label-free paper-based electrochemical impedance immunosensor for human interferon gamma detection

조회수 : 24 등록일 : 2018.11.23 00:00

저자 : Shin, K (Shin, Kwanwoo)
출처 : SENSORS AND ACTUATORS B-CHEMICAL
출판일 : 2019.01.15


Label-free paper-based electrochemical impedance immunosensor for human interferon gamma detection


Ruecha, N (Ruecha, Nipapan)1 ] Shin, K (Shin, Kwanwoo)2,3 ] Chailapakul, O (Chailapakul, Orawon)4 ] Rodthongkum, N (Rodthongkum, Nadnudda)1 ]


[ 1 ] Chulalongkorn Univ, Met & Mat Sci Res Inst, Bangkok, Thailand
[ 2 ] Sogang Univ, Dept Chem, Seoul, South Korea
[ 3 ] Sogang Univ, Inst Biol Interfaces, Seoul, South Korea
[ 4 ] Chulalongkorn Univ, Fac Sci, Dept Chem, Electrochem & Opt Spect Ctr Excellence, Bangkok, Thailand



Human interferon-gamma (IFN-gamma) plays an important role in diagnosis of tuberculosis susceptibility, which is one of the crucial health problems worldwide. Here, a novel platform of paper-based microfluidic device coupled with label-free electrochemical impedance immunosensor was developed for sensitive detection of human IFN-gamma. Polyaniline (PANI) was deposited on a graphene (G) screen-printed paper electrode by using cyclic voltammetry. The modified electrode provides high electrochemical conductivity and surface area for antibody immobilization, which significantly enhances the sensitivity. A linear relationship between impedance and logarithmic concentrations of human IFN-gamma was found in a range of 5-1000 pg mL(-1) with a detection limit (LOD) of 3.4 pg mL(-1). Interestingly, the PANI-G modified electrodes display 31-fold higher sensitivity compared to PANI-modified electrodes. The PANI-G modified electrode was successfully applied to the determination of human IFN-gamma in human serum with a good reproducibility. Moreover, the system offers several advantages including low cost, low sample volume requirement, disposability, and rapid analysis relative to traditional methods, allowing the platform to be used as an alternative tool for human IFN-gamma screening.






  • 목록보기
  • 이전글보기
  • 다음글보기