Publication

Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system

조회수 : 20 등록일 : 2018.10.15 00:00

저자 : Lee, KY (Lee, Keel Yong); Shin, K (Shin, Kwanwoo)
출처 : NATURE BIOTECHNOLOGY
출판일 : 2018.05.28


Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system


Lee, KY (Lee, Keel Yong)1,2,3,4,5 ] Park, SJ (Park, Sung-Jin)3,5 ] Lee, KA (Lee, Keon Ah)1,6 ] Kim, SH (Kim, Se-Hwan)1,6 ] Kim, H (Kim, Heeyeon)1,2 ] Meroz, Y (Meroz, Yasmine)7 ] Mahadevan, L (Mahadevan, L.)7,8 ] Jung, KH (Jung, Kwang-Hwan)1,6 ] Ahn, TK (Ahn, Tae Kyu)4 ] Parker, KK(Parker, Kevin Kit)3,5 ] Shin, K (Shin, Kwanwoo)1,2,5 ]


[ 1 ] Sogang Univ, Inst Biol Interfaces, Seoul, South Korea
[ 2 ] Sogang Univ, Dept Chem, Seoul, South Korea
[ 3 ] Harvard Univ, Disease Biophys Grp, Wyss Inst Biol Inspired Engn, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[ 4 ] Sungkyunkwan Univ, Dept Energy Sci, Suwon, South Korea
[ 5 ] Sogang Univ, Sogang Harvard Res Ctr Dis Biophys, Seoul, South Korea
[ 6 ] Sogang Univ, Dept Life Sci, Seoul, South Korea
[ 7 ] Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[ 8 ] Harvard Univ, Dept Phys, Wyss Inst Biolog Inspired Engn, Kavli Inst Nanobio Sci & Technol,Dept Organism &, Cambridge, MA 02138 USA



Inside cells, complex metabolic reactions are distributed across the modular compartments of organelles(1,2). Reactions in organelles have been recapitulated in vitro by reconstituting functional protein machineries into membrane systems(3-5). However, maintaining and controlling these reactions is challenging. Here we designed, built, and tested a switchable, light-harvesting organelle that provides both a sustainable energy source and a means of directing intravesicular reactions. An ATP (ATP) synthase and two photoconverters (plant-derived photosystem II and bacteriaderived proteorhodopsin) enable ATP synthesis. Independent optical activation of the two photoconverters allows dynamic control of ATP synthesis: red light facilitates and green light impedes ATP synthesis. We encapsulated the photosynthetic organelles in a giant vesicle to form a protocellular system and demonstrated optical control of two ATP-dependent reactions, carbon fixation and actin polymerization, with the latter altering outer vesicle morphology. Switchable photosynthetic organelles may enable the development of biomimetic vesicle systems with regulatory networks that exhibit homeostasis and complex cellular behaviors.






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