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Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

조회수 : 722 등록일 : 2018.04.12 00:00

CHEMICAL COMMUNICATION
Kim, S (Kim, Sanggil); Lee, HS (Lee, Hyun Soo) 2018.03.25

 
Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase
 
 
Kim, S (Kim, Sanggil)[ 1 ] ; Lee, HS (Lee, Hyun Soo)[ 1 ]
 
 
 
 
 
L-Dihydroxyphenylalanine (DOPA) was biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in Escherichia coli, and the biosynthesized amino acid was directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirmed the genetic incorporation of biosynthesized DOPA, and revealed its potential for various biochemical applications.
 
 
 
 
 
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