Publication

Application of Paper EWOD (Electrowetting-on-Dielectrics) Chip: Protein Tryptic Digestion and its Detection Using MALDI-TOF Mass Spectrometry

조회수 : 16 등록일 : 2017.07.20 00:00

저자 : Jang, I (Jang, Inae); Shin, K (Shin, Kwanwoo); Oh, HB (Oh, Han Bin)
출처 : BIOCHIP JOURNAL
출판일 : 2017.06.20
 
 
Application of Paper EWOD (Electrowetting-on-Dielectrics) Chip: Protein Tryptic Digestion and its Detection Using MALDI-TOF Mass Spectrometry
 
 
Jang, I (Jang, Inae)[ 1 ] ; Ko, H (Ko, Hyojin)[ 1 ] ; You, G (You, Gwangro)[ 1 ] ; Lee, H (Lee, Hyunji)[ 1 ] ; Paek, S (Paek, Seunghwi)[ 1 ] ; Chae, H (Chae, Heedo)[ 1 ] ; Lee, JH (Lee, Jae Hwan)[ 2 ] ; Choi, S (Choi, Sunkyung)[ 2 ] ; Kwon, OS (Kwon, Oh-Sun)[ 1 ] ; Shin, K (Shin, Kwanwoo)[ 1 ] ; Oh, HB (Oh, Han Bin)[ 1 ]
 
 
[ 1 ] Sogang Univ, Dept Chem, Seoul 04107, South Korea
[ 2 ] ADD, CBR Def Directorate, Yuseong 34186, Daejeon, South Korea

 
 
A paper-based open EWOD chip was used for protein tryptic digestion, and the resulting peptides were analyzed using MALDI-TOF mass spectrometry to identify the proteins. Although on-chip protein digestion was previously demonstrated on a glass-based EWOD platform, this is the first report to show that a paper-based EWOD can also be used for protein digestion and further analysis by mass spectrometry. A number of protein digestion processes, i.e., disulfide bond reduction, alkylation, buffering, and tryptic digestion, can be successfully conducted on the paper-based EWOD chip. Furthermore, a "Y"-shaped junction design was shown to be effective in successfully manipulating a droplet for protein digestion. A paper-based EWOD platform offers a cheap and versatile avenue for biological applications.
 
 
 
 
 
 
 
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