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TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode

조회수 : 235 등록일 : 2017.02.10 00:00

저자 : Jang, I (Jang, Inae) ; Moon, B (Moon, Bongjin) ; Oh, HB (Oh, Han Bin)
출처 : JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
출판일 : 2017.01.01
 
TEMPO-Assisted Free Radical-Initiated Peptide Sequencing Mass Spectrometry (FRIPS MS) in Q-TOF and Orbitrap Mass Spectrometers: Single-Step Peptide Backbone Dissociations in Positive Ion Mode
 
 
Jang, I (Jang, Inae)[ 1 ] ; Lee, SY (Lee, Sun Young)[ 2 ] ; Hwangbo, S (Hwangbo, Song)[ 1 ] ; Kang, D (Kang, Dukjin)[ 3 ] ; Lee, H (Lee, Hookeun)[ 4 ] ; Kim, HI (Kim, Hugh I.)[ 5 ] ; Moon, B (Moon, Bongjin)[ 1 ] ; Oh, HB (Oh, Han Bin)[ 1 ]
 
 
[ 1 ] Sogang Univ, Dept Chem, Seoul 04107, South Korea
[ 2 ] Kyung Hee Univ, Coll Pharm, Seoul 02447, South Korea
[ 3 ] Korea Res Inst Stand & Sci, Div Metrol Qual Life, Ctr Bioanal, Daejeon 34113, South Korea
[ 4 ] Gachon Univ, Gachon Inst Pharmaceut Sci, Inchon 21936, South Korea
[ 5 ] Korea Univ, Dept Chem, Seoul 02841, South Korea
 
 
The present study demonstrates that one-step peptide backbone fragmentations can be achieved using the TEMPO [2-(2,2,6,6-tetramethyl piperidine-1-oxyl)]-assisted free radical-initiated peptide sequencing (FRIPS) mass spectrometry in a hybrid quadrupole time-of-flight (Q-TOF) mass spectrometer and a Q-Exactive Orbitrap instrument in positive ion mode, in contrast to two-step peptide fragmentation in an ion-trap mass spectrometer (reference Anal. Chem. 85, 7044-7051 (30)). In the hybrid Q-TOF and Q-Exactive instruments, higher collisional energies can be applied to the target peptides, compared with the low collisional energies applied by the ion-trap instrument. The higher energy deposition and the additional multiple collisions in the collision cell in both instruments appear to result in one-step peptide backbone dissociations in positive ion mode. This new finding clearly demonstrates that the TEMPO-assisted FRIPS approach is a very useful tool in peptide mass spectrometry research.
 
 
 
 
 
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