Publication

Production of uracil from methane by a newly isolated Methylomonas sp SW1

조회수 : 193 등록일 : 2017.01.12 00:00

저자 : Bin Oh, H (Bin Oh, Han)
출처 : JOURNAL OF BIOTECHNOLOGY
출판일 : 2016.12.20
 
 
Production of uracil from methane by a newly isolated Methylomonas sp SW1
 
 
Kim, S (Kim, Sangwoo)[ 1 ] ; Lee, W (Lee, Wangjun)[ 1 ] ; Song, I (Song, Insu)[ 2 ] ; Kwon, Y (Kwon, Yuhyun)[ 1 ] ; Yun, S (Yun, Seokhun)[ 3 ] ; Park, S (Park, Soohyun)[ 1 ] ; Cho, S (Cho, Sukhyeong)[ 1 ] ; Oh, BK (Oh, Byung-Keun)[ 1 ] ; Bin Oh, H (Bin Oh, Han)[ 2 ] ; Lee, J (Lee, Jinwon)[ 1 ]
 
 
[ 1 ] Sogang Univ, Dept Chem & Biomol Engn, 35 Baekbeom Ro, Seoul 04107, South Korea
[ 2 ] Sogang Univ, Dept Chem, 35 Baekbeom Ro, Seoul 04107, South Korea
[ 3 ] CJ CheilJedang, Biotechnol Res Inst, 55 Gwanggyo Ro 42 Gil, Suwon 16495, South Korea
 
 
 
Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+ concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1 mg/L in 84 h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria. (C) 2016 Elsevier B.V. All rights reserved.
 
 
 
 
 
 
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