Publication

Direct protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins

조회수 : 138 등록일 : 2016.12.12 00:00

저자 : Kim, S (Kim, Sanggil) ; Lee, HS (Lee, Hyun Soo)
출처 : BIOORGANIC & MEDICINAL CHEMISTRY
출판일 : 2016.11.15
 
Direct protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins
 
 
Kim, S (Kim, Sanggil)[ 1 ] ; Ko, W (Ko, Wooseok)[ 1 ] ; Sung, BH (Sung, Bong Hyun)[ 2 ] ; Kim, SC (Kim, Sun Chang)[ 3 ] ; Lee, HS (Lee, Hyun Soo)[ 1 ]
 
 
[ 1 ] Sogang Univ, Dept Chem, Seoul 121742, South Korea
[ 2 ] KRIBB, Bioenergy & Biochem Res Ctr, Daejeon 305806, South Korea
[ 3 ] Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 305701, South Korea
 
 

Proteins often function as complex structures in conjunction with other proteins. Because these complex structures are essential for sophisticated functions, developing protein-protein conjugates has gained research interest. In this study, site-specific protein-protein conjugation was performed by genetically incorporating an azide-containing amino acid into one protein and a bicyclononyne (BCN)-containing amino acid into the other. Three to four sites in each of the proteins were tested for conjugation efficiency, and three combinations showed excellent conjugation efficiency. The genetic incorporation of unnatural amino acids (UAAs) is technically simple and produces the mutant protein in high yield. In addition, the conjugation reaction can be conducted by simple mixing, and does not require additional reagents or linker molecules. Therefore, this method may prove very useful for generating protein-protein conjugates and protein complexes of biochemical significance. (C) 2016 Published by Elsevier Ltd
 
 
 
 
 
 
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